Journal: Cerebral Cortex (New York, NY)
Article Title: Dopamine Receptor Expression Among Local and Visual Cortex-Projecting Frontal Eye Field Neurons
doi: 10.1093/cercor/bhz078
Figure Lengend Snippet: Distribution of dopamine receptors across inhibitory neuron populations across cortical layers. (A) Across all layers, the proportion of D2R-expressing parvalbumin+ neurons is higher than the proportion of D1R-expressing parvalbumin+ neurons. (B) There is no significant difference in the relative prevalence of D1Rs compared with D2Rs in calbindin+ neurons across layers. (C) There is no significant difference in the relative prevalence of D1Rs compared with D2Rs in calretinin+ neurons across layers. Significance was determined using chi-squared tests calculated on total counts. Parvalbumin+ N = 197, 44, 88, 75; P = 1.98 × 10−7, 3.89 × 10−5, 1.36 × 10−10, 4.65 × 10−9 for layers II, III, IV, V, and VI, respectively. Calbindin+ N = 232, 34, 46, 70; P = 0.64, 0.46, 0.61, 0.31 for layers II, III, IV, V, and VI, respectively. Calretinin+ N = 352, 31, 41, 52; P = 5.04 × 10−4, 0.42, 0.87, and 0.27 for layers II, III, IV, V, and VI, respectively. Bar heights and standard error bars are calculated from the across-animal mean proportions and the exact values are described in Table Table4.4. Animal Ns respectively for Parvalbumin+ D1R, D2R = 4, 3; Calbindin+ D1R, D2R = 4, 4; Calretinin+ D1R, D2R = 3, 4.
Article Snippet: DAPI stains DNA and is therefore a label for all nucleated cells. table ft1 table-wrap mode="anchored" t5 Table 3 caption a7 Antigen Host Vendor information Dilution D1R Rabbit polyclonal ADR-001; Alomone Labs, Jerusalem, Israel 1:200 D2R Goat polyclonal SC-7522; Santa Cruz Biotechnology, Santa Cruz, CA 1:200 D2R Rabbit polyclonal ADR-002; Alomone Labs, Jerusalem, Israel 1:200 Neurogranin Mouse monoclonal SC-514922; Santa Cruz Biotechnology, Santa Cruz, CA 1:200 SMI-32 Mouse monoclonal NE1023; Millipore, Temecula, CA 1:1000 Parvalbumin Mouse monoclonal P3088; Sigma-Aldrich Inc., St. Lous, MO 1:1000 Calbindin Mouse monoclonal CB300; Swant Inc., Switzerland 1:1000 Calretinin Mouse monoclonal 6B3; Swant Inc., Switzerland 1:1000 Somatostatin Rat monoclonal MAB354; Millipore, Temecula, CA 1:400 Open in a separate window Antibody information Imaging We identified the FEF as the rostral bank of the arcuate sulcus, posterior to the principal sulcus ( Moschovakis et al. 2004 ; Percheron et al. 2015 ) and performed tile scans of continuous areas of cortex (pial surface to white matter).
Techniques: Expressing
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